Guanidine isothiocyanate, an important raw material for in vitro diagnostic reagents

Guanidine isothiocyanate, GTC, is a white crystalline powder which is irritating,sensitive to light, soluble in ethanol and water, and has a melting point of 118 °C. It is used to denature lyse cells and extract RNA and DNA without RNase and DNase activity. In this epidemic, guanidine isothiocyanate played an important role as the main raw material for nucleic acid detection reagents.

Guanidine isothiocyanate is a decoupling agent and a powerful protein denaturant. During nucleic acid extraction, the main component of lysate is guanidinium isothiocyanate, which is a deodorizer. It is a strong protein denaturant, which can dissolve protein and make the secondary structure of protein disappear, leading to the degradation of cell structure, so that the nuclear protein is quickly separated from the nucleic acids.

Because guanidine isothiocyanate has no RNase and DNase activity, it is also used for sample storage. Guanidine isothiocyanate has the function of inhibiting Dnase / RNase activity, can save DNA and RNA from being degraded by enzymes, and protects tissues or cells.

Guanidine isothiocyanate is also used in fluorescence in situ hybridization to promote and promote fluorescence in situ hybridization. Fluorescence in situ hybridization (FISH) is a new molecular cytogenetic technology, which has the characteristics of high speed, strong detection signal, high hybridization specificity and multiple staining. It is widely used in the fields of virus infection analysis, human prenatal diagnosis, tumor genetics and genome evolution. According to the composition for promoting fluorescence in-situ hybridization, guanidinium isothiocyanate is added. Guanidinium isothiocyanate can promote DNA in a probe to be combined with DNA in a sample, and the hybridization efficiency is improved.

For in vitro diagnostic kits, the quality of raw materials, especially core raw materials, even determines the quality of products. Guanidine isothiocyanate is a raw material that plays a key role in many in vitro diagnostic reagents, especially the nucleic acid detection reagents used in the diagnosis of this epidemic. Yacoo science can provide good quality guanidine isothiocyanate products to ensure the diagnosis of epidemic The supply of reagent raw materials facilitates the development and use of in vitro diagnostic reagents.

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