F-Co(OH)2) based catalytic interface increase the luminol chemiluminescence system to a hundredfold

Chemiluminescence (CL) has the characteristics of high sensitivity, wide detection linear range and no pollution. It is an ideal immunoassay technique. In chemiimmunoluminescence analysis, the use of catalysts and luminescence enhancers can stabilize chemiluminescence, enhance its intensity and prolong luminescence time. In addition, designing a catalytic interface that preferentially attract reactants is also one of the important strategies to enhance chemiluminescence.

A flower-like cobalt hydroxide (f-Co(OH)₂) based catalytic interface with hierarchical and porous architecture were in situ created in the coexistence of BSA and Co²⁺ by Chinese scientists .It be used to boost the generation of reactive oxygen species (ROS) from dissolved O₂ molecule and can effectively enhance chemiluminescence.

At present, chemiluminescence immunoassay mainly includes luminol, isoluminol, acridine and cyclodioxane derivatives (such as CSPD, CDP-SATR, AMPPD, etc.) systems. 

Benefiting from the oxidase-like catalysis capability and the unique microstructure of f-Co(OH)₂, ROS was efficiently produced. Meanwhile, the capping ligands of BSA endowed the interface with the capability of enriching functionality through the interaction between BSA and luminol. 100-fold CL enhancement was achieved using the as-prepared catalytic interface compared with the classical luminol-Co²⁺ or luminol-BSA system.

Moreover, the proposed catalytic amplification mechanism could be extended to the different proteins such as lysozyme, protamine, thrombin, papain. Based on the quenching effect on CL, a sensitive sensing platform was constructed for the determination of ascorbic acid with satisfied results.

Related links: Luminol, BSA

Reference:

Huanhuan Xing. et al. In Situ Formed Catalytic Interface for Boosting Chemiluminescence.Analytical Chemistry. 2020.DOI: 10.1021/acs.analchem.0c02112