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Substance data and safety information of Tetramethylbenzidine (TMB 54827-17-7)

2014-08-06 来源:亚科官网
 
First, the substance data
English name: 3,3',5,5'-Tetramethylbenzidine
Alias: TMB
Molecular formula: C16H20N2
Molecular weight: 240.34
Structure:
 
Exterior:
 
Melting point: 168-171 °C (lit.)
Storage temperature: 2-8 ° C
Uses: This product is a non-carcinogenic substitute of benzidine (negative Ames test), suitable as a peroxidase substrate for enzyme-linked immunosorbent assay. The substrate produces a soluble light blue final product which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction (turns yellow) can be terminated with 2M H2SO4 and the data read at 450nm. A sensitive specific reagent for detecting blood, measuring hemoglobin and peroxidase.
 
Second, Safety information
Lable

Lable word

Warning

Dangerous 

H315-H319-H335

Warning 

P261-P305 + P351 + P338

Personal Protective Equipment 

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Hazard code (Europe)

Xi

Risk statement (Europe)

36/37/38

Safety statement (Europe)

26-36

WGK Germany 

3

Third, References

 

1Use of tetramethylbenzidine in plasma hemoglobin assay.

 

J C Standefer and D Vanderjagt
Clinical Chemistry, 23(4), 749-751 (1977)

Plasma hemoglobin can be assayed by substituting 3,3',5,5'-tetramethylbenzidine for benzidine (a carcinogen) as the chromogenic reducing substance. Sensitivity, precision, and accuracy compare well with methods in which benzidine is used. Plasma inh.

2Tetramethylbenzidine--a substitute for benzidine in hemoglobin analysis.

R C Lijana and M C Williams
Journal of Laboratory and Clinical Medicine, 94(2), 266-276 (1979)

Plasma hemoglobin is routinely measured by some modification of the benzidine-hydrogen peroxide method, particularly at low concentrations. Because benzidine is carcinogenic, and thus impractical for clinical use under current restrictions, the iden..

3Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3,3',5,5'-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine.

H H Liem et. al
Analytical Biochemistry, 98(2), 388-393 (1979)

43,3',5,5'-Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay.

E S Bos et. al
Journal of Immunoassay, 2(3-4), 187-204 (1981)

The use of 3,3',5,5' - tetramethylbenzidine as non-mutagenic chromogen for the end point determination in enzyme-immunoassay (EIA) is described. In sandwich EIAs for HCG and HBsAg and in a competitive EIA for testosterone, the colour yield with TMB .

5Quantitative determination of myeloperoxidase using tetramethylbenzidine as substrate.

P C Andrews and N I Krinsky
Analytical Biochemistry, 127(2), 346-350 (1982)

6[Horseradish peroxidase: kinetic studies and optimization of peroxidase activity determination using the substrates H2O2 and 3,3',5,5'-tetramethylbenzidine].

H Gallati and I Pracht
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 23(8), 453-460 (1985)

Horseradish peroxidase is often used as a labelling and indicator enzyme in enzyme immunoassays. In order to optimize the peroxidase activity determination, the kinetics of the catalytic reaction were investigated in relation to the concentration of.

7Loss of Endocan tumorigenic properties after alternative splicing of exon 2.

Florence Depontieu et. al
BMC Cancer, 8(1), 14 (2008)

Endocan was originally described as a dermatan sulfate proteoglycan found freely circulating in the blood. Endocan expression confers tumorigenic properties to epithelial cell lines or accelerate the growth of already tumorigenic cells. This molecul..

8 Immunogenicity of human mesenchymal stem cells in HLA-class I-restricted T-cell responses against viral or tumor-associated antigens.

Fabio Morandi et. al
Stem Cells, 26(5), 1275-1287 (2008)

 


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