Application of Tricine|CAS:5704-04-1| in the Field of Proteolysis

Tricine(CAS:5704-04-1)is an excellent amphoteric good's buffer. CN108251487B patent introduces a preparation method of Xinjiang black bee royal jelly gluten hydrolyzate with antioxidant activity. Royal jelly has many nutritional and health-care functions, especially strong anti-oxidation ability, and its protein component is the key to delay aging substance. Domestic research is more on soybean protein and whey protein, and there is no report on the research on royal jelly protein. The purpose of the present invention is to provide a kind of preparation method of Xinjiang black bee royal jelly gluten hydrolyzate with antioxidant activity, and its preparation method as follows:

1. Preparation of Xinjiang black bee royal jelly freeze-dried powder;

2. Degreasing and preparing the main protein of royal jelly;

3. Preparing globulin and obtaining raw materials for the preparation of gliadin;

4. Preparation of gliadin and raw materials for obtaining gluten;

5. Preparation of gluten: add Tricine to 0.01mol/L NaOH solution, the amount of Tricine is half of the amount of NaOH, and then add gluten to prepare raw materials to extract, filter, and dialyze the filtrate with a dialysis membrane , and the retentate is freeze-dried to obtain gluten.

6. Preparation of Xinjiang black bee royal jelly gluten hydrolyzate;

7. Separation of different molecular weight peptides in gluten hydrolysate.

Beneficial effects:

The gluten extracted under the optimal experimental strip accounts for 19.2% of the dry weight of royal jelly, which is very close to the theoretical value of 19.88±0.35. Xinjiang black bee royal jelly gluten has a certain antioxidant effect, the antioxidant activity of the product after enzymatic hydrolysis is greatly improved.

References

CN108251487B Preparation method of Xinjiang black bee royal jelly gluten hydrolyzate with antioxidant activity.