CAS：28836-03-5| The Application of ANS-NH4 in IVD

Product Name/English Name：8-Anilino-1-naphthalenesulfonic acid ammonium salt
English abbreviation：ANS-NH4
CAS：28836-03-5
Molecular Formula：        C16H16N2O3S
Article No.：    B0058
Structural Formula：

Product Introduction
ANS-NH4 is mainly used as a dye intermediate, such as in the production of weak acid dark blue 5R and weak acid black BR. In addition, ANS-NH4 can also be used to prepare dissociating agents.

Application of ANS-NH4
TT3 and TT4 are important components in the detection of clinical nail function projects. Currently, the commonly used detection reagents for the above two components in clinical practice mainly rely on the procurement of imported detection reagents. The main function of this detection reagent is to dissociate TT3 and TT4 in the bound state from their respective operating proteins, thereby becoming free molecules, to facilitate the detection of them by relevant reagents and improve the accuracy of clinical measurements. 99.7% of TT3 in human blood binds to running proteins, while 99.95% of TT4 reversibly binds to running proteins. This specific binding poses difficulties and challenges for the development of quantitative detection reagents for alphas. Therefore, when detecting TT3 and TT4 in serum, it is necessary to dissociate all binding molecules in order to obtain accurate levels of TT3 and TT4 in serum. At present, how to effectively dissociate TT3 and TT4 in the sample serum from the binding state into free molecules has become a challenge in experimental research and development. Therefore, the CN112362432B patent has developed a universal dissociating agent for TT3 and TT4 in serum and its preparation method. Its specific components are: metal chelates 0.01%~0.1%, N-heterocyclic compounds 0.01%~1.0%, stabilizers 0.05%~0.2%, surfactants 0.2%~1.0%, additives 0.5%~4.0%, and the rest are deionized water.
The auxiliary agent is a combination of one or both of 8-phenylamino-1-naphthalene sulfonic acid ammonium salt and sodium citrate. 8-phenylamino-1-naphthalene sulfonic acid ammonium salt has a high affinity for the hydrophobic surface of proteins and can bind to the protein surface in low polarity regions; Sodium citrate has excellent anticoagulation and buffering properties, and does not cause protein denaturation.
The dissociating agent of the present invention has good dissociation effect and can be used as a sample pretreatment solution in the detection reagent of alpha functional items. It can effectively dissociate the bound TT3 and TT4 in serum from their respective operating proteins, making them free. After becoming free, the content of free TT3 and TT4 is detected and verified using CLIA, making the sample results more accurate.

References
CN112362432B A universal dissociating agent for TT3 and TT4 in serum and its preparation method.