The New Type of Nano Enzyme That Imitates Peroxidase Activity for Sensitive Detection of Glucose

 A few days ago, researchers at the University of Science and Technology of China prepared a single iron center nanozyme (Fe SSN) dispersed on the surface of porous nitrogen-doped carbon, and designed an integrated hydrogel colorimetric biosensor, which successfully achieved the visual assessment of glucose And quantitative testing.

Diabetes is a serious hazard to human health, and effective in vitro blood glucose measurement and early screening of diabetic patients are of great significance in the active prevention and treatment of diabetes.

Nanozymes play an important role in detecting blood glucose in vitro. Nano-enzyme means that nano-materials have catalytic activity similar to natural enzymes, which can effectively catalyze various biochemical reactions. Compared with natural enzymes, nanozymes have the advantages of simple preparation, low price, good stability, tolerable harsh reaction conditions, and easy labeling and modification. According to published reports, there are currently more than 50 kinds of nanomaterials that can simulate the activity of natural enzymes such as glucose oxidase and catalase. The Fe SSN reported in this study has injected new vitality into the nanozyme family.

Fe SSN has a definite coordination structure and a high density of active centers, and exhibits outstanding peroxidase activity by effectively activating H₂O₂ into hydroxyl radicals (·OH). The researchers used 3,3',5,5'-tetramethylbenzidine (TMB) and other peroxidase substrate color-changing reactions to perform glucose colorimetric detection.

In this research ,a low-cost integrated agarose-based hydrogel colorimetric biosensor is designed and successfully achieves the visualization evaluation and quantitative detection of glucose. Moreover, it expands the application of single‐site catalysts in the fields of nanozyme‐based biosensors and personal biomedical diagnosis.

Related  link: 3,3',5,5'-tetramethylbenzidine

References: Min Chen, et al. Single Iron Site Nanozyme for Ultrasensitive Glucose Detection. Small, 2020. DOI: 10.1002/smll.202002343