Application of DTT |CAS:3483-12-3|in detection kits

DTT is a small molecule organic reducing agent. DTT is mainly used in nucleic acid extraction/protection, IVD kits, reduction of disulfide bonds in proteins, electroless nickel plating, etc. The editor of YACOO introduced the application of DTT in the detection kit of hepatitis B surface antibody.

Hepatitis B is a serious infectious disease. There are two common detection methods for hepatitis B: enzyme-linked immunosorbent assay and colloidal gold immunochromatography. The enzyme-linked immunosorbent assay is simple to operate, free of radioactive contamination, and low in price, but its sensitivity is low, and the accuracy and precision of the detection results are poor. Colloidal gold immunochromatography is fast, convenient, inexpensive, and the results are easy to interpret, but the sensitivity is too low. Therefore, it is necessary to invent a hepatitis B surface antibody detection kit with high sensitivity and specificity.

CN111707826A provides a hepatitis B surface antibody detection kit, which consists of magnetic particle-HBsAg recombinant antigen and alkaline phosphatase-labeled HBsAg recombinant antigen.

Magnetic particle-HBsAg recombinant antigen and HBsAg recombinant antigen in alkaline phosphatase-labeled HBsAg recombinant antigen were processed as follows: HBsAg recombinant antigen was pretreated with high concentration of DTT (10-20mmol/L), and the Dialysis and purification, dialyzed in a specific dialysate for 2h, at this time, the DTT concentration in the raw material was reduced to 1-5mmol/L.

Compared with the prior art, the hepatitis B surface antibody detection kit of the present invention has the same sensitivity as imported reagents, and has lower detection cost.

References

CN111707826A  Hepatitis B surface antibody detection kit