Application of D-trehalose|CAS:6138-23-4| in cell culture medium

D-trehalose （CAS:6138-23-4）is an organic compound with a molecular formula of C₁₂H₂₂O₁₁·2H₂O and a molecular weight of 378.3. Trehalose has stable biofilm and protein structure, and is mainly used in the field of biological product protection. Trehalose has the effect of inhibiting the decomposition of collagen, so it can be used to improve osteoporosis. In the CN111808799B patent, a method for culturing multifunctional stem cells and the medium used are introduced. The specific components of the medium are as follows:

Transferrin 0.6μg/mL, Stem Cell Growth Factor 7μg/mL, Epidermal Growth Factor 2μg/mL, L-Glutathione 25μg/mL, Cytoprotectant 0.3μg/mL, Growth Inducer 0.6μg/mL, aspartic acid 6μg/mL, ferric citrate 34 ng/mL, insulin 13μg/mL, and pantothenic acid 0.9μg/mL.

Cell protectant is composed of D-trehalose and hydroxyethyl starch in a mass ratio of 2:13.

Growth inducer was composed of chitooligosaccharide, sodium cholate and paeonol in a mass ratio of 10:5:1.

Molecular weight of chitosan oligosaccharide is 3000.

Beneficial effect

1.Cytoprotective agent compounded by D-trehalose and hydroxyethyl starch in this patent can not only reduce the damage of pluripotent stem cells, improve the activity of pluripotent stem cells, but also improve the proliferation ability of pluripotent stem cells. It is beneficial to maintain the shape of the pluripotent stem cells without changing, and ensure the purity of the obtained pluripotent stem cells.

2.Growth inducer in this patent, which is composed of chitosan oligosaccharide, sodium cholate and paeonol in a certain mass ratio, can significantly improve the in vitro culture efficiency of multifunctional stem cells, and it is easy to obtain a large number of multifunctional stem cells, which Overcomes the difficulty of cultivating pluripotent stem cells in large quantities.

References

CN111808799B A multifunctional stem cell culture method and medium used.