Application of TRIS-HCl|CAS：1185-53-1| in Biotechnology

TRIS-HCl (CAS: 1185-53-1) is an organic compound with molecular formula of C₄H₁₁NO₃ and molecular weight of 157.6. TRIS-HCl is an important biological buffer and can also be used as an organic synthesis intermediate. In addition, it can also be used for the growth of protein crystals under different pH conditions and the formation of intermediate fibers of nematode lamin. In addition, TRIS-HCl is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. In the CN109468311B patent, a bacterial lysate, a kit and a method for extracting plasmid DNA are disclosed. TRIS-HCl solution is widely used as a solvent for nucleic acids and proteins, and forms a part of the buffer system used for lysis. The specific technical proposal is:

A cell lysis solution for extracting plasmid DNA is composed of lysis buffer, lysozyme and RNase A. The lysis buffer is composed of 1.5～10g sucrose, 0.7～2.2g disodium ethylenediaminetetraacetate, 0.3～0.9g TRIS-HCl, 2.7～5.4g NH₄Cl, 0.1～ 2.0mL 0.5% Triton X-100, 0.2～0.6g CaCl₂, 0.5～8.0g guanidine hydrochloride, 0.5～ 3.0g guanidine isothiocyanate, and the rest is water, The pH of cell lysate is 5.0～ 8.0.

The unique bacterial lysate disclosed in this patent improves the lysate efficiency, reduces the pollution caused by protein and RNA, and also improves the binding efficiency of plasmid DNA and centrifugal adsorption column.

Reference

CN109468311B The invention relates to a bacterial lysate, a kit and a method for extracting plasmid DNA.