Application of TRIS-HCl|CAS：1185-53-1| in in vitro diagnostic technology

TRIS-HCl (CAS: 1185-53-1) is an organic compound with molecular formula of C₄H₁₁NO₃ and molecular weight of 157.6. TRIS-HCl is an important biological buffer and can also be used as an organic synthesis intermediate. In addition, it can also be used for the growth of protein crystals under different pH conditions and the formation of intermediate fibers of nematode lamin. In addition, TRIS-HCl is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. In the CN108008125A patent, a lyophilized working solution and immunomagnetic bead lyophilized product and their preparation method are introduced. TRIS-HCl in the patent lyophilized protective solution is mainly used as buffer solution. This week, YACOO editor introduced the specific composition of the immunomagnetic bead lyophilized working solution, as follows：

The freeze-drying working solution in this patent includes freeze-drying buffer solution, freeze-drying protective agent and preservative, and the mass ratio of the three substances is 1~10:1~500:1;

The lyophilized buffer is one or more combinations of TRIS-HCl buffer, PBS buffer, HEPES buffer and boric acid borax buffer;

Freeze drying protectants include sugars, BSA and mannitol. The carbohydrate is one or more combinations of trehalose, sucrose, lactose, glucose and maltose. The mass ratio of carbohydrate to BSA is 0.1~20:1, and the mass ratio of carbohydrate to mannitol is 0.1~20:1.

The preservative is Proclin 300.

The invention provides an immune magnetic bead freeze-drying agent, which can be stored for a long time, has the advantages of good stability, easy re dissolution, etc. Nowadays, the demand for TRIS-HCl in the domestic and foreign markets is very large. YACOO will continue to optimize the process while meeting the existing social needs to produce higher quality products to serve the society.

Reference

CN108008125A The invention relates to a lyophilized working solution and a lyophilized immune magnetic bead product and a preparation method thereof.