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Application of MOPS|CAS:1132-61-2| in Liquid Chromatography Tandem Mass Spectrometry

2022-11-29 来源:转载自第三方

MOPS is an organic compound with molecular formula of C7H15NO4S and molecular weight of 209.26. MOPS is an important biological buffer, which is often used to prepare RNA electrophoresis buffer. It can not only be used as a buffer system of cell culture medium, but also a common electrophoresis buffer and protein purification chromatography buffer. CN113820430A patent introduces a reagent used to detect antibacterial drugs and its preparation method. MOPS is used as a stabilizer to prepare calibrators or quality control products, thus facilitating the implementation of accurate detection of antibacterial drugs. The specific configuration method is as follows:

1.2% MOPS plasma solution and calibration solution and mixed quality control solution are mixed according to the ratio of 9:1 to obtain 1×Calibration solution and quality control solution;

2.Preparation of internal standard: weigh an appropriate amount of 16 kinds of antibiotic isotope internal standards, use appropriate solvent to dissolve and constant volume to obtain isotope internal standard stock solution with a concentration of 1mg/ml. Use 5% MOPS aqueous solution as the diluent, add the volume of each stock solution of the calculated volume according to the final use concentration of the internal standard of each antibacterial drug, and supplement the remaining volume with the diluent to obtain the internal standard;

3.Sample freeze-drying: Put the prepared calibration solution and quality control solution into the freeze-drying machine for vacuum freeze-drying, and put them into the reagent box.

The patent can effectively improve the stability of MOPS by using MOPS as the stabilizer to prepare quality control and calibrator, maintain the plasma matrix composition to the maximum extent, and simplify the transportation and storage process of calibrator and quality control. The calibration sample and quality control sample prepared by the invention are used for the liquid chromatography tandem mass spectrometry detection of antibacterial drugs, which can simultaneously ensure the detection of at least 16 drugs, significantly improve the detection efficiency, and have high responsiveness and good repeatability.


CN113820430A A reagent for detecting antibacterial drugs and its preparation method.