CAS：145224-94-8|The Application of MES·H2O in the Biomedical

2-(N-morpholine) ethanesulfonic acid monohydrate abbreviated as MES·H2O in English, with CAS 145224-94-8, is an organic compound with molecular formula C6H15NO5S and molecular weight 213.25. MES·H2O (CAS: 145224-94-8) is a biological buffer widely used in laboratory research and development and chemical and pharmaceutical synthesis. A preparation method for 3D printed porous biomimetic knee joint cartilage scaffold was developed in the CN113288521A patent. This week, YACOO editor will briefly introduce the preparation method of the porous biomimetic knee joint cartilage scaffold.
        1. Bone modeling: 3D scanning of the knee joint and the damaged area of the knee joint is performed using CT scanning equipment to obtain image data. Based on the damaged knee joint, the size and shape that can be transplanted are redesigned using 3D software.
        2.Preparation of 3D printing scaffold material: Extract fresh cartilage tissue, process it into 1*1* 1mm thin slices, rinse repeatedly with distilled water 3-5 times, sterilize and crush, add an appropriate amount of sterile triple distilled water at low temperature, and crush evenly. After   3 centrifugations and removing the supernatant, the acellular porcine cartilage extracellular matrix (ECM) slurry can be obtained. Take a certain amount of gelatin powder and dissolve it in PBS buffer to prepare a 200 g/L gelatin solution. Mix it with an equal volume epoxy grout (ECM grout) and add a certain amount of polylactic acid.
        3.Preparation of hyaluronic acid hydrogel: Dissolve 200 mg of hyaluronic acid powder in 15 mL of 2- (N-morpholine) ethanesulfonic acid hydrate solution, add 285-290 mg of N-hydroxysuccinimide, and then add 95 mg of propargylamine, dialysis for 2 h, and freeze dry to form a spongy solid. Take out 30-40mg of sponge like solid and dissolve it in 2-3mL of double distilled water. Dissolve 30mg of azide polyethylene glycol azide in 30μL dimethyl sulfoxide. Mix the above two solutions and add 10μL copper sulfate solution. Mix well before adding 10μLascorbic acid solution . Mix evenly to prepare hyaluronic acid hydrogel.  
        4.Printing using 3D printing technology

The above methods have solved the problems of low utilization of artificial cartilage scaffold components and insufficient strength, toughness, and wear resistance of the scaffold. The porous structure used can stimulate the repair and growth of bone cells, enabling the product to meet the affinity of human bones while reducing stress concentration and stress shielding of the implant. The customized printed product using 3D method is more in line with the characteristics of human cartilage, It not only ensures mechanical properties but also enables secondary bone growth.

Reference
CN113288521A A preparation method for 3D printed porous biomimetic knee joint cartilage scaffold.