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Introduction of Nitrotetrazolium Blue Chloride (NBT)

2015-08-07 来源:转载自第三方
Key words: detection reagent; NBT; alkaline phosphatase
Product Name: Nitrotetrazolium Blue Chloride (NBT)
CAS No.: 298-83-9
Item No.: L0037
Molecular formula: C40H30Cl2N10O6
Molecular weight: 817.636
Appearance and smell: yellow powder
Content / Specification: ≥98.0%
Uses: Dehydrogenase and other peroxidase substrates, also one of the alkaline phosphatase substrates
Storage conditions: 2-8 ° C refrigeration, protected from light
It is a substrate for nitrotetrazolium chloride (NBT) dehydrogenase and other peroxidases. A precipitating reagent for the determination of glucose-6-phosphate dehydrogenase and blood coagulation factor fibrinogen. Also one of the alkaline phosphatase substrates, an insoluble blue product is formed under the catalysis of alkaline phosphatase. NBT can also be used as a method of screening for urinary tract infections. It is commonly used in immunohistochemistry (IHC), immunocytochemistry (ICC) and Western blot (WB) experiments.

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