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APS-5(193884-53-6)应用介绍
APS-5 of ELISA AP Substrate is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of APS-5 provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus.
Material information:
中文名称:
9-(4-氯苯硫代磷酰氧亚甲基)-10-甲基-9,10-二氢化吖啶二钠盐;
(4-氯苯巯基)(10-甲基-9,10-二氢化吖啶亚甲基)磷酸二钠盐
英文名称:[(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)methanol 1-(dihydrogen phosphate) disodium salt;
Lumigen APS 5;
APS 5
分子式:C21H15ClNNa2O4PS
分子量:489.82
Cas: 193884-53-6
following advantages:
Excellent sensitivity - low picogram to femtogram
Rapid peak intensity - reduced assay time and increased throughput,The light-output peak intensity of Lumigen APS-5 reaches sustained maximum within seconds of substrate addition. Light emission is maximal at 450 nm.
Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control
Sustained luminescence - timing is less critical with light intensities able to be read at any time to produce linear calibration curves