2,3,5-Triphenyltetrazolium chloride method of preparation, action mechanism and Application

 A, preparation methods: take the red tetrazolium (TTC) 0.1 grams, add distilled water 10 ml of solution, 10 pounds 30 minutes sterilization (not with mediummixed after sterilization but independent sterilization, other medium is sterilizedbefore use according to the required amount added).

Two, the mechanism of red tetrazolium: combined with triphenyltetrazolium chloride and hydrogen colorless can generate the red material, while the hydrogen production is closely related with the dehydrogenation enzyme,dehydrogenation enzyme is closely related with the biological activity of TTC, sooften used in the determination of biological activity or distinguish betweenbiological and non biological such as colony and food residue.

Three, triphenyltetrazolium chloride used in many places, the most commonly used is the seed activity, another plant disease detection, detection of totalcolonies live and tissue activity etc.