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Indole -3- carbinol (700-06-1) on radiation sensitivity of lung carcinoma cells EGFR dependent regulation
2014-08-02
来源：亚科官网
Background and purpose: indole -3- carbinol (indole-3-carbinol, I3C) is a kind of cruciferous vegetables in the main effective chemical plants, and has anti-cancer and anti-cancer effect. The purpose of this study was to observe whether the I3C effect of epidermal growth factor receptor (epidermal growth factor receptor, EGFR) expression in lung cancer cells with different levels of radiation sensitivity.
Methods: using MTT and clone formation assay were used to detect the survival rate and growth of lung cancer cells; siRNA transfection method to reduce the expression of EGFR protein in cells; expression of EGFR protein and mRNA were determined by Western blot and RT-PCR respectively.
Results: the 5mol/L dose has no obvious toxic and side effect of I3C pretreatment significantly reduced the expression of EGFR positive of radiosensitivity of human lung adenocarcinoma H1975 and human lung squamous cell carcinoma H226 cells to gamma ray irradiation, and I3C on the expression of EGFR radiation sensitive negative human lung squamous cell carcinoma NIH-H520 cells is very small. Western blot results showed that I3C could increase the expression level and Y845 phosphorylation of H1975 and EGFR protein in H226 cells. EGFR siRNA decreased the expression of EGFR protein in NIH-H1975 cells, increase the radiosensitivity of the cells, and effectively reduce and suppress I3C induced cell radiation effect.
Conclusion: Our results are the first to confirm that I3C can regulate EGFR expression and phosphorylation level affects the radiosensitivity of lung cancer cells, suggesting that EGFR may be an important target protein sensitivity of radiotherapy in lung cancer I3C.
