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Benserazide hydrochloride (14919-77-8) determination by high performance liquid chromatography
2014-08-04
来源：亚科官网
Method name: - high performance liquid chromatography determination of Benserazide Hydrochloride.
Scope of application: content of the chromatographic determination of Benserazide Hydrochloride high performance liquid.
This method is suitable for Benserazide hydrochloride.
Method: the samples and internal standard were made into the mobile phase solution, into the high performance liquid chromatography was applied in chromatographic separation, detector using UV absorption, at the detection wavelength is 220nm Benserazide Hydrochloride (C10H15N3O5 · HCl) and internal standard of dopamine hydrochloride absorption value, calculate the content of.
Reagent: 1 methanol
2 three trifluoroacetic acid
Equipment: 1 instruments
1.1 high performance liquid chromatography
The 1.2 column
Eighteen alkyl silane bonded silica as filler, the number of theoretical plates should not be less than 1000 is calculated by Benserazide Hydrochloride peak, Benserazide Hydrochloride peak and internal standard substance peak separation shall conform to the requirements.
1.3 ultraviolet absorption detector
2 chromatographic conditions
Mobile phase: 2.1 water three trifluoroacetic acid + methanol =1000 120
2.2 the detection wavelength: 220nm
The 2.3 column temperature: room temperature
Sample preparation: 1 weighing samples
Take this product 15mg, accurately weighed.
2 internal standard solution preparation
The amount of dopamine hydrochloride, adding dilute methanol solution is every 1mL contains about 7Mg, which was.
3 sample solution preparation
The samples containing 50mL Brown volumetric flask, adding internal standard solution precision 5mL, with the mobile phase diluted to the scale, shake, to obtain the solution for test.
4 standard solution preparation
Precision weighing Benserazide Hydrochloride controlled amount of goods, and the sample with the method.
Note: the "precision weighing" system should be accurate to refer to the weight of the weight of 1/1000. "The precise amount" means the amount of volume accuracy should be in accord with the national standard of the volumetric pipettes accuracy requirements.
Operation steps: the precise amount of the test solution and the reference solution of 10 μ L was injected into the HPLC, detector using ultraviolet absorption at 220nm wavelength, determination of Benserazide Hydrochloride and dopamine hydrochloride absorption value, according to the internal standard method was calculated by peak area, namely
