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Brief description : the application of CAPSO biological buffer

2020-04-20 来源:亚科官网

 Biological buffers play an important role in biochemical research, in vitro diagnosis, etc. The CAPSO introduced in this article is a commonly used and important buffer. CAPSO,3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, Its CAS number is 73463-39-5, and Its appearance is a white powder. CAPSO is soluble in water with a pH buffer range of 8.9-10.3 and a pKa value of 9.6 at 25℃.

CAPSO is commonly used in immunoblotting and immunoprecipitation experiments, and is widely used in the preparation of enzymatic reactions and detection reagents. According to patent CN110488000A , CAPSO can be prepared the lysis solution for extracting membrane protein. It can prevent a large change in pH, and can maintain the ionic strength in a physiological range and prevent the protein from binding to other ions to form an insoluble product.

CAPSO as a biological buffer in some enzyme detection reagents can not only provide a stable and suitable pH environment for enzymatic reactions, but also provide a good storage environment for the reagents, which is conducive to stable and effective storage of reagents.

According to published literature, CAPSO can be used in creatine kinase detection kits, aspartate aminotransferase detection reagents, and leucine aminopeptidase detection reagents, and is of great significance for the prevention and diagnosis of liver diseases, myocarditis and other diseases.

Yacoo Science is a national high-tech enterprise specializing in "IVD Reagents Raw Materials", "New Electronic and Chemical Materials", "Biobuffers" and "Pharmaceutical Intermediates". Biological buffering agent is one of the main products of Yacoo Science.It is a class of special-purpose alcohol-ammonia fine chemicals. It does not participate in or interfere with biochemical processes. In addition to CAPSO, Yacoo's advantageous products also includes TRIS, HEPES, MOPS, CAPS, etc. They are widely used in life science research, in vitro diagnostics, biopharmaceuticals, biobeauty, paints and other fields. And the quality is recognized and trusted by customers in various industries.

Related links: CAPSO, TRIS, HEPES, MOPS, CAPS

 



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