The Application Of TOOS In IVD Testing Items

TOOS, 3-(N-ethyl-methylanilino)-2-hydroxy propane sulfonic acid, Sodium Salt is a in vitro diagnostic reagent，which used widely. And it is the most commonly used in the new Trinder's reagent.TOOS is used in many clinical biochemical testing items.

TOOS is widely used in diagnostic tests and biochemical tests through Trinder reaction. Trinder reaction, also known as "coupling end point colorimetry", is based on the principle that hydrogen peroxide (H₂O₂) produced by the enzyme action of the tested substance can generate red quinone imine compound in the presence of 4-aminoantipyrine (4-AAP) and peroxidase (POD).

Blood Glucose Testing Items: TOOS is used to prepare glucose testing reagents and glycated albumin testing reagents in blood glucose metabolism items.

According to the description of patent CN105572065A, glucose oxidase is used to catalyze the oxidation of glucose to hydrogen peroxide, and platinum-bismuth nano-simulated peroxidase catalyzes the oxidation of 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS) coupling color development, the color of the color product solution is purple, with the increase of glucose concentration, the solution With the addition of color, the maximum absorption wavelength of the color rendering system is 590 nm, and the glucose content can be obtained from the absorbance at 590 nm.

Liver Function Routine Examination Items: Adenosine deaminase (ADA) activity is a sensitive indicator reflecting liver injury and is one of the routine examination items of liver function. TOOS and bovine serum albumin, ethylenediaminetetraacetic acid disodium and other adenosine deaminase detection reagents have The color rendering effect is good, the reaction is fast, stable, and the precision of measurement is high.

In addition, TOOS is also used in diagnostic reagents for blood lipid testing items such as triglycerides and cholesterol testing items, and has important value in clinical diagnosis.

Related links: TOOS

References:

Glucose detection kit based on platinum-bismuth nano mimic peroxidase.CN105572065A