Application of Guanidine Isothiocyanate|CAS:593-84-0| in nucleic acid detection

In 2019, COVID-19 broke out.COVID-19 can cause acute infectious pneumonia. The virus is extremely spread. In order to prevent and control the virus, nucleic acid testing is required. In the process of nucleic acid detection, the throat swab, nasal swab or specific part of the person to be tested shall be collected as the sample to be tested. Achieving rapid nucleic acid extraction is one of the key steps to shorten the clinical nucleic acid diagnosis time. When the number of samples to be tested is too large, or the samples to be tested are collected in areas without testing conditions, the sample must be stored or transported to the testing point. Carry out subsequent nucleic acid extraction, purification and clinical experiments. In the process of storage or transportation, the RNA in the sample to be tested is easily degraded, resulting in undetectable RNA, resulting in "false negative" nucleic acid detection.

In the existing technology, isotonic saline solution or phosphate buffer is generally used as the virus protectant. After the sample to be tested is placed in the virus protectant prepared by isotonic saline solution or phosphate buffer, it must be kept at low temperature and cannot be repeated Freeze-thaw; in the actual application process, dry ice is used to maintain the low temperature when the sample to be tested is transported or transferred over long distances.

CN112708655A provides a protective agent capable of storing RNA viruses at room temperature. The protective agent includes: guanidine isothiocyanate, sodium citrate, proteinase K, RNase inhibitor, DNA degrading enzyme and sterile water. Each 10mL protective agent includes: Guanidine thiocyanate 0.2g-0.9g, sodium citrate 0.1g-0.8g, proteinase K 1g-8g, RNase inhibitor 0.2g, DNA degrading enzyme 15ku, the balance is sterile water.

The RNA virus protective agent provided by the method can preserve the RNA virus at room temperature, thereby avoiding repeated freezing and thawing of the RNA virus during storage and transportation, and effectively preventing RNA degradation.

References

CN112708655A RNA virus protective agent and preparation method of protective agent.