Application of D-trehalose|CAS:6138-23-4| in the field of reproductive medicine

D-trehalose is an organic compound with a molecular formula of C₁₂H₂₂O₁₁·2H₂O and a molecular weight of 378.3. Trehalose has stable biofilm and protein structure, and is mainly used in the protection of biological products, such as genetically engineered enzymes and other enzymes, cell membranes, antibodies, antigens, organelles, etc. In the patent CN105746494B, a human sperm cryoprotectant containing D-trehalose and a preparation method thereof are introduced, the purpose is to solve the technical problems of decreased sperm motility and low recovery rate existing in the existing human sperm slow cryoprotectant , the technical solutions adopted are as follows:

A human sperm cryoprotectant containing D-trehalose, each 100ml of the human sperm cryoprotectant contains the following components: glucose 1.5-1.6g, trisodium citrate dihydrate 1.3g, glycine 1.3g, Glycerin 20ml, egg yolk 20ml, D-trehalose 2.0-2.5g and deionized water 60ml.

Beneficial effect

1. This cryoprotectant can effectively improve the movement speed and movement index of human sperm after resuscitation, and has a significant beneficial effect on improving the recovery rate of human sperm freezing and improving the qualified rate of sperm donation for human sperm bank volunteers.

2. The cryoprotectant can effectively improve the SOD activity of frozen semen samples, which reflects the improvement of the ability of semen to scavenge oxygen free radicals.

References

CN105746494B A kind of human sperm cryoprotectant containing D-trehalose and preparation method thereof.