Application of D-trehalose |CAS:6138-23-4|in the field of biological testing

D-trehalose (CAS: 6138-23-4) is an organic compound with a molecular formula of C₁₂H₂₂O₁₁·2H₂O and a molecular weight of 378.3. Trehalose has stable biofilm and protein structure, and is mainly used in the protection of biological products, such as genetically engineered enzymes and other enzymes, cell membranes, antibodies, antigens, organelles, etc.

A kind of PCT calibrator buffer solution is introduced in CN111896754A patent. This buffer solution does not need to be lyophilized, and the stability of the calibrator is still good in the liquid state, and its technical scheme is as follows:

The PCT calibrator buffer solution in this patent includes animal protein, anionic surfactant, 4-aminoantipyrine and D-trehalose.

Animal protein: The animal protein is bovine serum albumin or casein, and the concentration is ≥0.01g/mL.

Anionic surfactant: the anionic surfactant is sodium lauryl sulfate, and the volume concentration is 0.15%-0.30%.

4-aminoantipyrine: The concentration is 0.02-0.04 mg/mL.

D-trehalose: The concentration is 0.02-0.04 mg/mL.

In this patent, the addition of animal protein to the buffer solution can improve the stability of the buffer solution, and the addition of anionic surfactants, 4-aminoantipyrine and D-trehalose can further improve the stability; the buffer solution does not need to be lyophilized , the stability of the calibrator is still good in the liquid state.

References

CN111896754A A kind of PCT calibrator buffer solution.