Application of BES |CAS：10191-18-1|in material synthesis

BES, CAS: 10191-18-1, is an organic compound with molecular formula of C6H15NO5S and molecular weight of 213.25. BES is a biological buffer, widely used in laboratory research and development and chemical and pharmaceutical synthesis. In addition, BES can also be used in material synthesis. The CN101723389B patent introduces a preparation method of magnetic silica microspheres with surface modified cation. The specific preparation steps are as follows:
(1) Preparation of magnetic silica microspheres;
(2) Epoxidation of magnetic silica microspheres;
(3) Cationic surface modification:
(a) Mix the magnetic silica microsphere with epoxy-bonded surface prepared in step (2) and 0.01 mol/L dilute hydrochloric acid, and heat the reaction while stirring;
(b)After the reaction is completed, magnetic separation is performed to obtain the precipitate, and the precipitate is washed with secondary water;
(c)Dissolve the cationic modifier（Histidine, polyhistidine, imidazole or its derivatives, 2 - (N-morpholino) ethanesulfonic acid or BES）in ultrapure water, adjust the pH value to 7~10, add the precipitate washed in step (3. b), stir evenly, and heat the reaction;
(d)After the reaction is completed, magnetic separation is carried out to obtain the precipitate. The precipitate is washed with secondary water, and then washed with pre-treatment solution, and then put into pre-treatment solution to prepare magnetic silica microspheres with surface modified cation.
The magnetic silica microsphere with surface modified cation prepared by the above method, because the modified cation is a kind of compound with pKa value between 4 and 8, it does not need high concentration of salt solution to elute when using it to extract nucleic acid, so it does not need to use ethanol precipitation to remove high concentration of salt, which saves time and effort, and does not cause the loss of nucleic acid in the process of ethanol precipitation.

Reference
CN101723389B Preparation of magnetic silica microspheres with surface modified cation.