CAS：145224-94-8|Application of MES·H2O in detection kits

Clenbuterol is a type of β-Stimulant, commonly known as "clenbuterol".It can increase growth rate, accelerate fat conversion and decomposition, and increase the lean meat rate of pork after consuming clenbuterol in pigs. Therefore, it is called clenbuterol.If a person ingests the drug excessively or without illness, there may be side effects or poisoning accidents. According to Announcement No. 235 of the Ministry of Agriculture of China, clenbuterol and its salts and ester compounds shall not be detected in all food animals and all edible tissues. Announcement No.176 of the Ministry of Agriculture stipulates that clenbuterol is prohibited from being used in feed and animal drinking water.
      The residual amount of clenbuterol is usually detected by instrumental analysis, such as HPLC, GC, GC-MS, etc. Due to the use of purification devices for sample pre-treatment using the above detection methods, and the tedious purification steps, multiple organic solvents are required. Therefore, the CN105301234B patent developed a clenbuterol immunomagnetic bead separation and enrichment kit, which consists of three parts: a magnetic bead coupled with clenbuterol monoclonal antibody, a complex solution, and a magnet.
      Magnetic beads coupled with clenbuterol monoclonal antibodies are prepared by mixing clenbuterol monoclonal antibodies with magnetic beads and coupling them in (N-morpholine) ethanesulfonic acid monohydrate (CAS: 145224-94-8).
      The complex solution contains bovine serum albumin, Tween-20, and NaN3 in phosphate buffer or trimethylaminomethane buffer.
      The magnet generates an external magnetic field, causing the magnetic beads coupled with clenbuterol monoclonal antibodies to aggregate on the magnet, achieving the purpose of separating the magnetic beads.
       2-(N-morpholine) ethanesulfonic acid monohydrate abbreviated as MES·H2O in English, with CAS 145224-94-8, is an organic compound with molecular formula C6H15NO5S and molecular weight 213.25. MES·H2O (CAS: 145224-94-8) is a biological buffer widely used in laboratory research and development and chemical and pharmaceutical synthesis. YACOO can provide customers with high-quality services of MES·H2O from gram to ton.

Reference
CN105301234B A Clenbuterol Immunomagnetic Bead Separation and Enrichment Kit and Its Application.