CAS：193884-53-6| The Application of APS-5 in Immunoassay

Product Name/English Name：[(4-chlorophenyl)sulfanyl-(10-methylacridin-9-ylidene)methyl] phosphate   
English abbreviation：APS-5
CAS：193884-53-6
Molecular Formula：C21H15ClNO4PS Na2
Article No.：Y0361
Structural Formula：

Product Introduction
APS-5 is a chemiluminescent substrate based on 9,10-dihydroacridine. The 9,10-dihydroacridine structure in APS-5 can continuously, stably, and efficiently emit light under the action of AP. After mixing APS-5 with ALP, the alkaline phosphatase phosphate radical is hydrolyzed, and the substrate immediately undergoes a decomposition reaction to release photons. Within a specific concentration range of alkaline phosphatase, the number of photons released is proportional to the concentration of alkaline phosphatase in the solution. Therefore, it can be used for quantitative detection of alkaline phosphatase and the production of chemiluminescence antibody detection kits. It has the highest detection sensitivity, stable detection results, and good repeatability, It has been promoted and used in medicine.
Application of APS-5
Chemiluminescence is a phenomenon of light radiation accompanying a chemical reaction of a substance. According to the different systems and labeling methods of chemiluminescence reactions, luminescent immunoassay can be broadly divided into three types: chemiluminescence immunoassay, chemiluminescence enzyme immunoassay, and bioluminescence immunoassay. Chemiluminescence enzyme immunoassay technology is used to label antigens or antibodies with enzymes in a certain reaction. After the immune reaction, luminescent reagents are added to measure the luminescence intensity of the luminescent system for antigen or antibody determination. CN103344633A patent provides a chemiluminescence substrate solution for alkaline phosphatase, which contains APS-5 120mg/L, N, N dimethylacridine nitrate 3.4mg/L, sodium dodecyl sulfate 1g/L, sodium sulfite 10mg/L, TWEEN-20 0.31g/L, and 0.26mol/L Tris buffer system.
The chemiluminescence substrate liquid has increased luminescence intensity, short entry time into the platform, and strong sensitivity.

References
CN103344633A A Chemiluminescence Substrate Solution for Alkaline Phosphatase.